放射免疫分析(RIA)自60年代问世以来,在生物医学各领域广泛应用,但放射性污染问题,一直困扰着人们。80年代末国外建立了化学发光技术,使之替代RIA成为可能,但其发光信号时间短,增加仪器测量的难度。酶放大学发光(CLEIA)是在化学发光的基础上加入碱性磷酸酶,延长发光信号的时间,使发光技术成为更趋成熟的超微量体外测量方法。本文就我院使用CLEIA检测游离三碘甲腺原氨酸(FT3)、游离四碘甲腺原氨酸(FT4)、灵敏促甲状腺激素 Radioimmunoassay (RIA) has been widely used in all fields of biomedicine since its introduction in the 1960s, but radioactive contamination has always plagued people. In the late 1980s, chemiluminescence technology was established abroad to make it possible to replace RIA. However, the luminescence signal time is short, which increases the difficulty of measuring instruments. Enzyme Luminescence (CLEIA) is the addition of alkaline phosphatase on the basis of chemiluminescence to extend the time of the luminescent signal, making luminescence technology a more mature ultra-micro in vitro measurement method. In this study, we used CLEIA to detect free triiodothyronine (FT3), free tetraiodothyronine (FT4), sensitive thyroid stimulating hormone