探讨了尿蛋白测定方法,并改良了CBB—I尿蛋白定量法,于试剂中加入SDS和TritcX-100,可改善不同比例白、球蛋白反应灵敏的差异。并观察了蛋白成份及温度对5种蛋白测定方法反应的影响。实验证明:CBB—Ⅱ法在许多方面优于其他方法,操作简单、快速,适于临床常规尿蛋白定量测定。 The urinary protein determination method was discussed, and CBB-I urinary protein quantitation method was improved. SDS and TritcX-100 were added to the reagent to improve the sensitivity of different ratios of white and globulin. The effect of protein composition and temperature on the reaction of five protein assays was also observed. Experiments show that: CBB-Ⅱ method in many ways better than other methods, simple, rapid, suitable for clinical routine urinary protein quantitative determination.