目的:通过静脉注入供体可溶性组织相容性抗原,诱导受体淋巴细胞凋亡。方法:应用3mol/L氯化钾的抽提供体(Wistar大鼠)可溶性抗原,然后将其供体可溶性抗原经静脉注入受体(SD大鼠),采用TUNEL法标记受体胸腺及脾脏淋巴细胞以观察其凋亡情况,受体淋巴细胞对供体抗原的反应采用混合淋巴细胞培养。结果:在体外,制备的供体可溶性抗原可刺激致敏后的受体淋巴细胞增生;在体内,供体可溶性抗原可导致受体胸腺及脾脏的淋巴细胞凋亡。结论:制备的供体可溶性抗原具有免疫学活性,能在体内诱导淋巴细胞凋亡。 OBJECTIVE: To induce lymphocyte apoptosis in recipient by intravenous infusion of donor soluble histocompatibility antigen. Methods: Soluble antigen of donor (Wistar rat) was extracted with 3mol / L potassium chloride, then its donor soluble antigen was injected intravenously into the recipient (SD rat), TUNEL method was used to label the recipient thymus and spleen lymphocytes To observe the apoptosis, the response of recipient lymphocytes to donor antigens using mixed lymphocyte culture. RESULTS: In vitro, donor soluble antigen was prepared to stimulate sensitized recipient lymphocyte proliferation. In vivo, donor soluble antigen could induce lymphocyte apoptosis in thymus and spleen of recipient. Conclusion: The prepared donor soluble antigen is immunologically active and can induce lymphocyte apoptosis in vivo.