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稻瘟病是水稻产区的主要病害之一,而稻瘟病菌的分离和产孢是进行稻瘟病研究的主要基础工作。许多农业科学工作者做过多种试验。日本的高坂淖尔,山崎义人等提出以下几种分离法:①病穗保湿单孢分离法,其中又分排菌法和稀释分离法;②罹病组织分离法。他们还进行了各种培养基、利用不同波长的光照促进孢子形成和用不同消毒液进行表面消毒的尝试。国内,罗宽等也比较成功地做了罹病组织分离的试验(未发表);李桦进行了稻瘟病菌培养技术的研究。本试验在于从中探讨一个理想的稻瘟病菌的分离和产孢方法。
Rice blast is one of the main diseases in rice producing areas. Isolation and sporulation of the rice blast fungi are the main foundation for the research on rice blast. Many agricultural scientists have done many experiments. Japan’s Sakamoto Seoul, Yoshio Yamazaki and other proposed the following separation methods: ① spike moisture moisture single spore separation method, which is divided into bacteria and dilution method; ② diseased tissue separation method. They also experimented with various media, using different wavelengths of light to promote sporulation and surface disinfection with different disinfectants. In China, Luo et al. Also succeeded in the study of the diseased tissue separation (unpublished); Li Hua conducted the research on the culture technology of Magnaporthe grisea. This experiment is to explore an ideal method for the isolation and sporulation of Magnaporthe grisea.