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自从Coons(1950)成功地运用异氰酸基荧光素与抗体球蛋白结合以来,已迅速地在医学和微生物学领域中广泛的应用开来。目前动物体內的抗原物质,抗体的形成以及确定不同待检材料(如涂片,组织切片)中的病原微生物等研究工作,常采取荧光抗体技术。为传染病的早期诊断提供了确切的线索。十几年来在荧光素方面也有了不少改进,其中性能较好最常应用的是异硫氰酸基荧光素。目前文献中的合成路线是:
Coons (1950) has been rapidly used in medicine and microbiology since the successful use of fluorescein conjugates with antibody globins. At present, the antigenic substances in animals, the formation of antibodies and the determination of pathogenic microorganisms in different materials to be tested (such as smear, tissue sections) and other research work, often take fluorescent antibody technology. For the early diagnosis of infectious diseases provides the exact clues. More than ten years in the fluorescein also has been a lot of improvement, which is the most commonly used performance is isothiocyanate fluorescein. The current synthetic route in the literature is: