论文部分内容阅读
目的:研究血清N-C、HA、LN水平与肝纤维化临床诊断的关系。方法:应用放射免疫分析法(RIA),对142例各型肝病患者血清进行联合检测Ⅳ型胶原(N—C)、透明质酸(HA)、层粘连蛋白(LN)含量并进行分析。结果:肝硬化、酒精性肝病患者血清N-C含量高于正常人群含量3倍以上者占75.0％、66.6％,在急性病毒性肝炎、慢性病毒性肝炎(轻度)患者高于正常值的2～3倍占92.3％、68.4％,但无超过3倍以上者。HA检测值>800ng/ml,肝硬化组占75％,慢性肝炎(中～重度)占31.58％。LN检测在各组肝病中增高,94.74％～100％患者为正常值的2～3倍,但各组间无明显差异。结论:N-C检测含量HA>正常值的3倍及>800ng/ml时特异性较强,可作为一界限范围。LN含量检测对判断肝纤维化程度的评价有待进一步累积资料。
Objective: To study the relationship between serum N-C, HA, LN and clinical diagnosis of liver fibrosis. Methods: Serum samples from 142 patients with liver disease were detected by radioimmunoassay (RIA). The contents of type Ⅳ collagen (N-C), hyaluronic acid (HA) and laminin (LN) were detected and analyzed. Results: The serum levels of NC in patients with liver cirrhosis and alcoholic liver disease were 75.0% and 66.6%, respectively, higher than those in the normal population by more than 3 times. In patients with acute viral hepatitis and chronic viral hepatitis (mild) Times accounted for 92.3%, 68.4%, but no more than 3 times more. HA test value> 800ng / ml, cirrhosis group accounted for 75%, chronic hepatitis (moderate to severe) accounted for 31.58%. LN test increased in each group of liver disease, 94.74% to 100% of patients with normal 2 to 3 times, but no significant difference between the groups. CONCLUSIONS: N-C levels> 3 times normal and> 800ng / ml are specific and can be used as a cut-off. LN content testing to judge the degree of liver fibrosis evaluation needs further accumulation of information.