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miRNA是一类动、植物细胞中负责转录后调控的非编码RNA,植物的miRNA通常来源于具有茎环结构的单链RNA前体上,加工成熟后同ARGONAUTE转变成蛋白复合体结构,通过结合并沉默蛋白合成模板的方式关闭完整的基因表达网络。由于miRNA存在组织表达的特异性,且miRNA在植物中的表达高度保守。通过高通量测序获得的红花(Carthamus tinctorious L.)miRNA序列信息与红花转录EST数据库比对,通过靶基因预测筛选,对属于104个家族的173个红花miRNA进行预测,其中得到109个miRNAs对应调控的385个红花靶基因。并且通过Nr基因注释表明多数红花miRNA的靶基因编码包括调控细胞生长发育、信号转导及新陈代谢等相关的功能蛋白。
MiRNAs are a class of non-coding RNAs responsible for post-transcriptional regulation in animal and plant cells. The miRNAs of plants usually originate from single-stranded RNA precursors that have a stem-loop structure. After processing, ARGONAUTE is converted into a protein complex structure by binding And silence the way the protein synthesis template closes the entire gene expression network. Due to the specificity of tissue expression of miRNA, miRNAs are highly conserved in plants. The Carthamus tinctorius L. miRNA sequence information obtained by high-throughput sequencing was compared with the safflower transcript EST database, and 173 safflower miRNAs belonging to 104 families were predicted by target gene prediction screening, and 109 A miRNAs corresponding regulation of 385 Saffron target genes. And through the Nr gene annotation, most of the safflower miRNA target genes encoded include regulating cell growth and development, signal transduction and metabolism and other related functional proteins.