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在pH5．75～6．4的条件下，云南基孔肯雅病毒及国外原型株能与鸽、鹅、鸡、鸭、绵羊和人O型红细胞发生凝集，尤以鸽、鹅红细胞为佳，凝集最适pH为5.75。在4℃、室温和37℃血凝反应无明显差别。交互血凝抑制试验表明，云南基孔肯雅病毒及国外原型株与同亚组的MAY、SF、GET和SIN病毒反应较弱（≤1：20），与西尼罗、乙脑、登革、科萨努尔森林病、兰加特、波瓦生和流行性出血热病毒无反应。结果表明，血凝抑制试验是基孔肯雅病毒的诊断和流行病学调查的较好方法。
Under the conditions of pH5.75 ~ 6.4, Yunnan Chikungunya virus and foreign prototype strains can agglutinate with pigeons, geese, chickens, ducks, sheep and human O-type erythrocytes, especially pigeons and goose red blood cells, The optimum pH for agglutination is 5.75. At 4 ° C, there was no significant difference in hemagglutination between room temperature and 37 ° C. The reciprocal hemagglutination inhibition test showed that the Yunnan Chikungunya virus and the foreign prototype strains reacted weakly with the MAY, SF, GET and SIN viruses of the same subgroup (≤1: 20), and were resistant to West Nile, JE, Dengue , The Caucus noir forest disease, Langat, bovis and the epidemic hemorrhagic fever virus. The results show that the hemagglutination inhibition test is a good method for the diagnosis and epidemiological investigation of Chikungunya virus.