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周永超(鞍钢劳动卫生研究所)报道:血液碳氧血红蛋白微量定量法,原理是利用连二亚硫酸钠(Na_2S_2O_4),将被检血液中的氧合血红蛋白(HbO_2)、微量高铁血红蛋白(MHb)转变成Hb,使血中仅存HbCO和Hb。HbCO及Hb的吸收光谱不同,通过分光光度计测定两峰处的消光度,经公式处理,算出HbCO的百分浓度。原法是统一先放还原剂,加血后放置10～15分钟,比色。本文不同之处是:统一先加缓冲液。加血后分批加
Zhou Yongchao (Ansteel Institute of Labor and Health) reported: blood carboxyhemoglobin micro quantitative method, the principle is the use of sodium dithionite (Na_2S_2O_4), will be seized in blood oxygenated hemoglobin (HbO_2), trace methemoglobin (MHb) into Hb, so that only the blood HbCO and Hb. The absorption spectra of HbCO and Hb are different, and the extinction of the two peaks is determined by a spectrophotometer, and the percentage concentration of HbCO is calculated by the formula. The original law is to put the reducing agent first unified, add blood after placing 10 to 15 minutes, colorimetric. The difference of this article is: Uniform first buffer. Add blood after the batch plus