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DNA结合蛋白与特异性DNA顺序的相互作用是基因转录调控的一种机制.对类固醇激素受体等基因调节蛋白的鉴定,有助于我们了解DNA-蛋白质相互作用,以及基因激活与抑制的控制.人糖皮质激素受体(hGR)的cDNA顺序已测出,有趣的是,这类激素受体顺序分析结果显示,其与鸟类成红细胞病毒v-erb-A癌基因产物p75~(gag-v-orb-A)蛋白质具有同源性.在此基础上,C.Weinberger和J.Sap两家实验室同时提出:c-erb-A基因可编码一种甲状腺素受体,或者说c-erb-A蛋白质是甲状腺素的一种高亲和力受体.实验证明:(1)c-erb-A与类固醇激素受体的完全结构同源性,说明erb-A可能是一种配基反应性调节蛋白;(2)癌基因表达蛋白和天然受体一样,与天然及合成的甲状腺素均具有亲和力;(3)erb-A体外转译
The interaction of DNA-binding proteins with specific DNA sequences is a mechanism of gene transcriptional regulation.The identification of gene regulatory proteins such as steroid hormone receptors helps us to understand DNA-protein interactions and the control of gene activation and repression . The cDNA sequence of human glucocorticoid receptor (hGR) has been determined. Interestingly, the sequence analysis of these hormone receptors showed that the cDNA sequence of avian corticosteroid receptor (hGR) was associated with avian erythrocytic virus v-erb-A oncogene product p75 ~ -v-orb-A) protein.On this basis, C.Weinberger and J.Sap two laboratories also proposed: c-erb-A gene can encode a thyroxine receptor, or c -erb-A protein is a high-affinity receptor for thyroxine.Experiments show that: (1) The complete structural homology between c-erb-A and the steroid hormone receptor indicates that erb-A may be a ligand reaction Regulatory proteins; (2) Oncogene-expressed proteins, like natural receptors, have affinity for both natural and synthetic thyroxins; (3) In vitro translation of erb-A