方法传代培养的人羊膜FL细胞进入对数生长期后,于无血清培养基中加入互隔交链孢霉提取物(简称261-B_2-3)处理,各组的终浓度分别为0、10~(-3)、10~(-2),10~(-1)mg/ml。在处理的第0、8、24和48小时各处理组各取一组细胞消化,制成单细胞悬液 Methods The cultured human amniotic membrane (FL) cells entered the logarithmic growth phase and were treated with Alternaria alternata extract (referred to as 261-B_2-3) in serum-free medium. The final concentrations of each group were 0, ~ (-3), 10 ~ (-2), 10 ~ (-1) mg / ml. At the 0th, 8th, 24th and 48th hour of treatment, each treatment group took a group of cells to digest to make a single cell suspension