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应用PCR 扩增方法检测203 例NIDDM 患者和165 例正常对照者血管紧张素(ACE)基因的16 内含子中287bp 片段缺失/插入多态性。结果显示，糖尿病肾病患者组ACE 基因缺失纯合型(DD)频率明显高于非肾病组和正常对照组，P<0.001；DD 缺失纯合型患者肾病进展速度明显快于缺失/插入(DI)杂合型及插入/插入(II)纯合型糖尿病患者，P<0.01。表明ACE 基因缺失纯合型是糖尿病肾病的独立危险因素之一，并可作为NIDDM 肾病发生发展的有效预测指标。
PCR amplification was used to detect the deletion / insertion polymorphism of 287bp fragment in 16 introns of angiotensin (ACE) gene in 203 patients with NIDDM and 165 healthy controls. The results showed that the frequency of DD gene deletion in patients with diabetic nephropathy was significantly higher than that in non-nephropathy patients and normal controls (P <0.001). The rate of nephropathy progression was significantly higher in patients with DD deficiency than that in patients with deletion / Heterozygous and insert / insert (II) homozygous diabetic patients, P <0.01. The results suggest that the lack of homozygosity of ACE gene is an independent risk factor of diabetic nephropathy and can be used as a valid predictor of the development of NIDDM nephropathy.