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去甲斑蝥酸钠(10μg/ml)处理非正常二倍体细胞(CHO)、人宫颈癌细胞(HeLa),48小时杀伤率分别为91.8％和58.3％;对正常二倍体小儿包皮细胞杀伤率仅7.7％,呈现有区别的杀伤。去甲斑蝥酸钠严重干扰CHO和HeLa细胞的有丝分裂,造成M期阻滞,CHO和HeLa细胞的最高有丝分裂指数(MI)分别高达60.0％和20.0％;染色体粘连成团,胞质分裂受阻,致使细胞形成多核,其多核率高达44.0％(CHO)和15.5％(HeLa)。这种染色体畸形和细胞多核化是CHO细胞死亡增加的主要原因。该药对小儿包皮细胞有丝分裂干扰较小,M期持续时间略有延长,最大有丝分裂指数值5.0％,多核率1.5％(与对照相同),死亡率低。去甲斑蝥酸钠对正常二倍体细胞(小儿包皮细胞)和非正常二倍体细胞(CHO)、肿瘤细胞(HeLa)的有丝分裂干扰程度不同,导致了“区别杀伤”。
After treatment with nocodanoic acid (10μg / ml), the diploid cells (CHO) and human cervical cancer cells (HeLa) were treated with 91.8% and 58.3% cytotoxicity respectively. The rate of only 7.7%, showing a difference between the killing. Sodium norcantharidinate seriously interfered the mitosis of CHO and HeLa cells, resulting in arrest in M phase. The highest mitotic index (MI) of CHO and HeLa cells were 60.0% and 20.0%, respectively. Chromosomal adhesions into clusters resulted in disruption of cytokinesis The cells formed multi-nuclei with multi-core rates of up to 44.0% (CHO) and 15.5% (HeLa). This chromosomal abnormality and cell multinucleation are the major causes of increased CHO cell death. The drug has less interference with mitosis in children’s foreskin cells, with a slightly longer duration of M phase, a maximum mitotic index value of 5.0% and a polynuclear rate of 1.5% (same as the control) with low mortality. Norcantharidin causes different degrees of mitotic interference to normal diploid cells (pediatric) and non-normal diploid cells (CHO) and tumor cells (HeLa), resulting in “differential killing”.