用细胞涂片作抗原进行免疫细胞化学染色,其阳性清晰度,对比度都不如组织切片。原因是涂片细胞干燥时,易固缩而造成细胞着色后结构不清,以及涂片中所含死细胞、细胞碎片、蛋白质成份可引起高度背景着色,给单细胞应用于免疫细胞化学技术及单克隆抗体筛选造成一定困难。采用细胞盖片培养做免疫细胞化学染色效果很好,不仅可以通过洗涤去除死细胞、碎片、蛋白质而消除背景着色,而且还能使细胞舒展,着色后的细胞轮廓、结构较清晰、但盖片培养时,因盖 Cell smears as antigens for immunocytochemical staining, the positive clarity, contrast is not as good as tissue sections. The reason is that when the smear cells are dry and easy to deflate, the cells are unclear in structure after being colored, and dead cells, cell debris and protein components contained in the smears can cause a high degree of background coloration, and the single cells are used in immunocytochemistry and Monoclonal antibody screening caused some difficulties. Immunocytochemical staining using cell cover sheet is very effective. It not only can remove dead cells, debris and protein by washing, but also can make the cells stretch. The colored cell outline and structure are clearer. However, Culture, due to cover