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分子标记的种间通用性可降低其开发成本,提高利用效率,促进遗传研究较薄弱物种的分子遗传学研究。为开发黄芪SSR分子标记,本研究利用大豆的66对基因组SSR(G-SSR)和43对EST-SSR引物在黄芪基因组中进行通用性分析,并选出其中条带清晰易辨的23对引物对6种不同来源的黄芪进行遗传分析。结果表明:大豆G-SSR、EST-SSR引物在黄芪中的通用性比例分别为31.82%、76.74%,多态性引物分别占18.18%、53.49%,大豆EST-SSR在黄芪的通用性高于基因组SSR,且通用的大豆SSR标记可以用于不同来源黄芪的遗传多样性分析。本研究发掘的多态性SSR引物可以有效用于黄芪的分子遗传多样性研究。
Interspecificity of molecular markers can reduce their development costs, improve utilization efficiency, and promote molecular genetic studies of weaker species in genetic research. In order to develop the SSR molecular marker of Astragalus, 66 pairs of genomic SSR (G-SSR) and 43 pairs of EST-SSR primers were used for the general analysis of Astragalus mongholicus genome and 23 pairs of primers Genetic analysis of Astragalus from six different sources. The results showed that the universal ratios of G-SSR and EST-SSR primers in Astragalus were 31.82% and 76.74%, respectively. The polymorphic primers accounted for 18.18% and 53.49% Genomic SSR, and common soybean SSR markers can be used for genetic diversity analysis of Astragalus from different sources. The polymorphism SSR primers discovered in this study can be effectively used for molecular genetic diversity of Astragalus.