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采用免疫组化ＡＢＣ法检测肿瘤抑制基因Ｐ５３、Ｐ１６蛋白在各级胃粘膜病变中的表达变化，以加深对胃癌发生分子学基础的认识。结果：萎缩性胃炎即有Ｐ５３蛋白阳性反应，且随着病变的加重表达率升高；Ｐ１６蛋白在正常胃粘膜中表达率最高，随着病变发展，其免疫阳性率呈下降趋势；此外两者在癌组织中的表达呈显著性相关（Ｐ＜０．０１），全部５０例癌组织中，检出Ｐ５３阳性表达组织２６例，其中同时伴有Ｐ１６阳性反应的组织１７例（１７／２６），伴Ｐ１６表达缺失的组织９例（９／２６），前者高于后者（Ｐ＜０．０５）。结果提示：肿瘤抑制基因Ｐ５３、Ｐ１６的异常表达发生于胃粘膜癌变早期，胃癌组织中Ｐ５３基因异常可能对Ｐ１６蛋白表达有反馈调节作用
Immunohistochemical ABC method was used to detect the expression of tumor suppressor genes P53 and P16 in gastric mucosal lesions at all levels to deepen the understanding of the molecular basis of gastric cancer. Results: Atrophic gastritis was positive for P53 protein, and the expression rate of P53 protein increased with the increase of pathological changes. The expression rate of P16 protein was the highest in normal gastric mucosa. The immunopositivity rate of P16 protein decreased with the development of lesions. (P <0.01). Of the 50 cases of cancerous tissues, 26 cases were positive for P53 expression, 17 cases (17/26) were positive for P16 positive reaction, 9 cases (9/26) with the absence of P16 expression, the former was higher than the latter (P <0.05). The results suggest that the abnormal expression of tumor suppressor genes P53 and P16 occurs in the early stage of carcinogenesis of gastric mucosa and the abnormal expression of P53 in gastric cancer may have feedback regulation on the expression of P16