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AIM:To investigate the expression of NF-κBp65 proteinand human telomerase reverse transcriptase (hTERT) andtheir correlation in gastric cancer and precancerous lesions.METHODS:Forty-one patients with primary gastric cancer,15 with dysplasia,23 intestinal metaplasia and 10 with normalgastric mucosa were included in this study.Expression ofNF-κBp65 protein,hTERT mRNA and protein were determinedby immunohistochemistry and in situ hybridization.RESULTS:The rate of p65 expression in normal gastricmucosa,intestinal metaplasia,dysplasia and carcinoma was0%,34.78%,53.33% and 60.98%,respectively,while therate of hTERT mRNA expression was 10.00%,39.13%,66.67% and 85.37% and the rate of hTERT protein expressionwas 0%,30.43%,60.00% and 78.05%,respectively.Allthe three parameters were significantly increased in dysplasiaand carcinoma compared to normal mucosa,while theexpression levels were also significantly higher in carcinomathan in intestinal metaplasia (P<0.05).In gastric cancertissues,nuclear staining rates of p65 and hTERT proteinwere both significantly associated with the degree ofdifferentiation,lymph node metastasis,clinical stage andinvasion depth (P<0.05).However,hTERT mRNA expressionwas only significantly associated with clinical stage.Therewas a positive correlation between p65 and hTERT mRNA(r_s=0.661-0.752,P<0.01),and between hTERT protein andhTERT mRNA (r_s=0.609-0.750,P<0.01).CONCLUSION:NF-κBp65 and hTERT expressions areupregulated at the early stage of gastric carcinogenesis.NF-κB activation may contribute to hTERT expression andthereby enhance telomerase activity,which represents animportant step in carcinogenesis progress.
AIM: To investigate the expression of NF-κBp65 protein and human telomerase reverse transcriptase (hTERT) and the correlation in gastric cancer and precancerous lesions. METHODS: Forty-one patients with primary gastric cancer, 15 with dysplasia, 23 intestinal metaplasia and 10 with normal gastric mucosa were included in this study. Expression of NF-κB p65 protein, hTERT mRNA and protein were determined in immunohistochemistry and in situ hybridization. RESULTS: The rate of p65 expression in normal gastric mucosa, intestinal metaplasia, dysplasia and carcinoma was 0%, 34.78%, 53.33% and 60.98%, respectively, while the rate of hTERT mRNA expression was 10.00%, 39.13%, 66.67% and 85.37% and the rate of hTERT protein expression was 0%, 30.43%, 60.00% and 78.05%, respectively. in dysplasia and carcinoma compared to normal mucosa, while theexpression levels were also significantly higher in carcinomathan in intestinal metaplasia (P <0.05) .In gastric cancertissues, nucleus ar staining rates of p65 and hTERT proteinwere both significantly associated with the degree of differentiation, lymph node metastasis, clinical stage and depth of invasion (P <0.05) .However, hTERT mRNA expression was only significantly associated with clinical stage. There was a positive correlation between p65 and hTERT (r_s = 0.661-0.752, P <0.01), and between hTERT protein and hTERT mRNA (r_s = 0.609-0.750, P <0.01) .CONCLUSION: NF-κBp65 and hTERT expressions are upregulated at the early stage of gastric carcinogenesis. κB activation may contribute to hTERT expression and athereby enhance telomerase activity, which represents animportant step in carcinogenesis progress.