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目的:探讨AnnexinⅡ在人肾透明细胞癌和癌旁组织样本中的表达情况及其临床意义。方法:采用Western blot方法检测29例新鲜肾透明细胞癌及相应癌旁组织中AnnexinⅡ蛋白的表达;采用免疫组织化学方法检测120例肾透明细胞癌及相应癌旁组织中AnnexinⅡ的表达情况,并将结果与患者的临床病理参数及预后进行相关性分析。结果:AnnexinⅡ在肾透明细胞癌及相应癌旁组织中均有表达,前者的表达水平较后者显著升高(P<0.01);免疫组织化学显示肾透明细胞癌组织中,AnnexinⅡ的阳性表达率高达67.5%,与癌旁组织存在明显差异(P<0.01);AnnexinⅡ在肾透明细胞癌组织中以胞膜表达为主,其表达状况与肾透明细胞癌患者的临床分期(P<0.05),组织学分级(P<0.05),肾被膜受侵(P<0.01)和远地转移(P<0.01)紧密相关;并且AnnexinⅡ的表达状况与肾透明细胞癌患者的5年生存率显著相关。结论:AnnexinⅡ在肾透明细胞癌组织中过表达,其与肾癌的发展密切相关,在肾癌的侵袭及转移过程中可能发挥重要作用。
Objective: To investigate the expression of Annexin Ⅱ in human renal clear cell carcinoma and adjacent normal tissue samples and its clinical significance. Methods: AnnexinⅡprotein expression was detected by Western blot in 29 cases of fresh renal clear cell carcinoma and its corresponding paracancerous tissues. Immunohistochemistry was used to detect the expression of AnnexinⅡ in 120 cases of clear cell carcinoma of renal cell and corresponding paracancerous tissues. The results were correlated with the clinicopathological parameters and prognosis of the patients. Results: Annexin Ⅱ was expressed in both of renal clear cell carcinoma and corresponding paracancerous tissues, the former was significantly higher than the latter (P <0.01). Immunohistochemistry showed the positive rate of Annexin Ⅱ in renal clear cell carcinoma (P <0.01). The expression of Annexin Ⅱ was mainly in the cytoplasm of renal clear cell carcinoma, and its expression was correlated with the clinical stage of renal clear cell carcinoma (P <0.05) The histological grade (P <0.05), renal capsule involvement (P <0.01) and distant metastasis (P <0.01) were closely related. The expression of Annexin Ⅱ was significantly associated with the 5-year survival rate of renal clear cell carcinoma. Conclusion: Annexin Ⅱ is overexpressed in renal clear cell carcinoma tissues. It is closely related to the development of renal cell carcinoma and may play an important role in the invasion and metastasis of renal cell carcinoma.