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缺乏有效的早期诊断方法是导致肝细胞癌(hepatocellular carcinoma,HCC)预后极差的主要原因之一.蛋白质异常糖基化与恶性肿瘤细胞侵袭、转移等生物学过程关系密切,人体内至少有50%的蛋白质发生了糖基化修饰.本实验采用IgY12去除血清高丰度蛋白、多植物凝集素亲和层析技术分别从20例肝癌和年龄、性别匹配的20例非癌慢性肝病患者血清中纯化N-连接糖蛋白、二维电泳分析差异表达的蛋白质斑点,质谱检测、生物信息学等技术鉴定了18个差异表达的糖蛋白和/或其异质体(12种高表达和6种低表达).ExPASy数据库比对结果表明,本实验鉴定的糖蛋白质分子含有至少1个已报道的N-糖基化位点.这些差异表达的糖蛋白属于急性期反应蛋白,分别具有蛋白酶抑制、生物转运、凝血和纤溶等功能,表明肝癌的发生发展过程中机体产生的急性期反应物可能是潜在的肝癌血清标志物.
The lack of effective early diagnosis is one of the main reasons leading to poor prognosis of hepatocellular carcinoma (HCC) .The abnormal protein glycosylation is closely related to the biological processes such as invasion and metastasis of malignant tumor cells, and there are at least 50 % Of the proteins were glycosylated.In this study, IgY12 was used to remove serum high abundance protein, and phytohemagglutinin affinity chromatography was performed on 20 cases of hepatocellular carcinoma and 20 cases of non-cancer chronic liver disease matched by age and gender 18 differentially expressed glycoproteins and / or their isoforms (12 high-expression and 6 low-expression) were identified by purification of N-linked glycoprotein, differentially expressed protein spots by two-dimensional electrophoretic analysis, mass spectrometry detection and bioinformatics techniques Expression) .ExPASy database comparison results show that the experimental identification of glycoprotein molecules containing at least one reported N-glycosylation sites.These differentially expressed glycoproteins belong to the acute phase reaction protein, respectively, with protease inhibition, biological Transport, coagulation and fibrinolysis and other functions, indicating that the occurrence and development of liver cancer in the body during acute phase reactant may be a potential serum markers of liver cancer.