论文部分内容阅读
目的白木香树在自然条件下不产生树脂,以自然、微生物或人工方式可使之形成沉香树脂。“小孔滴注法”接种镰刀菌人工诱导白木香结香,8个月后野外采集白木香结香部位(分泌黑色树脂的树干组织),提取其总RNA,为阐明沉香特征产物的代谢途径、揭示人工诱导白木香结香的分子机制奠定基础。方法 “小孔滴注法”人工造香,手工割取白木香结香部位。分别采用CTAB-Li Cl法、Trizol试剂法、RN09试剂盒法、改良异硫氰酸胍-CTAB法和Qiagen试剂盒法提取总RNA并检测完整性。结果与其它四种提取方法相比,Qiagen试剂盒法提取的RNA条带清晰,OD260/OD280比值在1.8~2.0的范围内。结论 Qiagen试剂盒法适用于镰刀菌人工诱导白木香结香部位总RNA提取,对其它次生代谢物质含量较高的植物中提取总RNA具有一定的借鉴意义。
Purpose White wood tree does not produce resin under natural conditions, natural, micro-organisms or artificial way to make the incense resin. Inoculation of Fusarium gracilis artificial induction of white woody incense sticks, 8 months after the wild collected white wood Xiangzixiang (secreted black resin trunk), extract the total RNA, in order to clarify the characteristics of the product Metabolic pathway, revealing the molecular mechanism of artificial induction of white woody incense lay the foundation. Method “hole drip method ” Artificial incense, hand-cut off the white woody incense parts. The total RNA was extracted by CTAB-Li Cl method, Trizol reagent method, RN09 kit method, modified guanidine isothiocyanate-CTAB method and Qiagen kit method, respectively. Results Compared with the other four extraction methods, the RNA bands extracted by Qiagen kit method were clear and the ratio of OD260 / OD280 was in the range of 1.8-2.0. Conclusion The Qiagen kit method is suitable for the total RNA extraction of the Fusarium graminearum induced by Fusarium spp., And has certain reference value for the extraction of total RNA from other plants with high secondary metabolites content.