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本文对牛蒡子中的化学成分进行了研究。采用多种柱色谱方法并结合制备型HPLC的方法分离纯化牛蒡子中的化学成分,进一步利用IR、UV、CD、MS、HR-ESI-MS、1D和2D NMR等技术对分离得到的化合物进行结构分析和鉴定。从牛蒡子80%乙醇提取物中分离鉴定了10个化合物,分别为:(7R,8R)-4,7,9,9’-tetrahydroxy-3,3’-dimethoxy-8-4’-oxyneolign-7’-ene-9’-O-β-D-glucopyranoside(1)、(7R,8R)-4,7,9,9’-tetrahydroxy-3,3’-dimethoxy-8-O-4’-neolignan-9’-O-β-D-glucopyranoside(2)、(7R,8R)-4,7,9,9’-tertahydroxy-3,3’-dimethoxy-8-4’-oxyneolignan(3)、(7S,8R)-dihydrodehydrodiconiferylalcohol-4-O-β-D-glucopyranoside(4)、(7S,8R,7’R,8’R)-pinoresinol-4,4’-di-O-β-D-glucopyranoside(5)、(8S,7’S,8’R)-4,4’,9’-trihydroxy-3,3’-dimethoxy-7’,9-epoxylignan-7-oxo-4’-O-β-D-glucopyranoside(6)、1-O-β-D-xylopyranosyl-(1→6)-O-β-D-glucopyranoside-2-methoxy-4-hydroxyphenol(7)、1-O-β-D-xylopyranosyl-(1→6)-O-β-Dglucopyranoside-3-methoxy-4-hydroxyphenol(8)、4-O-β-D-xylopyranosyl-(1→6)-O-β-D-glucopyranoside-4-hydroxy-3-methoxybenzylalcohol(9)和2-phenethylβ-primeveroside(10)。其中化合物1为一个新的8-O-4’型木脂素,化合物2~10均为首次从牛蒡子中分离得到。
In this paper, the chemical composition of Arctium was studied. The chemical constituents of Arctium lappa L. were isolated and purified by a variety of column chromatography methods combined with preparative HPLC. The isolated compounds were further characterized by IR, UV, CD, MS, HR-ESI-MS, 1D and 2D NMR techniques Structural analysis and identification. Ten compounds were isolated and identified from 80% ethanol extract of Arctium lappa, which were: (7R, 8R) -4,7,9,9’-tetrahydroxy-3,3’-dimethoxy-8-4’-oxyneolign- 7’-ene-9’-O-β-D-glucopyranoside (1), (7R, 8R) -4,7,9,9’-tetrahydroxy- (2), (7R, 8R) -4,7,9,9’-tertahydroxy-3,3’-dimethoxy-8-4’-oxyneolignan (7S, 8R) -dihydrodehydrodiconiferylalcohol-4-O-β-D-glucopyranoside (4), (7S, 8R, 7’R, 8’R) -pinoresinol-4,4’-di-O-β- glucopyranoside (5), (8S, 7’S, 8’R) -4,4 ’, 9’-trihydroxy-3,3’-dimethoxy-7’, 9-epoxylignan-7- oxo-4’-O- D-glucopyranoside (6), 1-O-β-D-xylopyranosyl- (1 → 6) -O-β-D-glucopyranoside- (1 → 6) -O-β-Dglucopyranoside-3-methoxy-4-hydroxyphenol (8), 4-O-β-D-xylopyranosyl- -hydroxy-3-methoxybenzylalcohol (9) and 2-phenethylβ-primeveroside (10). Compound 1 was a new 8-O-4 ’lignan. Compounds 2 ~ 10 were isolated from Arctium lappa for the first time.