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从力学生物学的角度探索生理层流剪切力(shear stress,SS)对树突状细胞(dendritic cells,DCs)的形态、细胞骨架和免疫表型分子表达水平的影响。采用常规方法从CD14~+单核细胞诱导获得未成熟DCs(immature DCs,im DCs)和成熟DCs(mature DCs,m DCs),旋转锥板装置给DCs加载10 dyn/cm~2的剪切力,观察DCs形态和细胞骨架的变化,流式细胞术和实时荧光定量PCR技术检测DCs表面分子CD80、CD83和CD86在蛋白和基因水平上的表达情况。在流体剪切力的作用下,DCs的细胞直径变小(p<0.05),细胞骨架(F-actin)发生了明显的重组。DCs的免疫表型分子CD80、CD83和CD86在蛋白和基因水平上的表达均受到影响。因此,DCs能够对生理层流剪切力作出应答,生理层流剪切力可能是DCs免疫功能的一个负调控因子,支持“力学免疫学(mechanoimmunology)”和“免疫力学生物学(immunomechanobiology)”的学术观点,这对于深入理解DCs的免疫调节功能来说具有重要意义。
The effects of physiological shear stress (SS) on the morphology, cytoskeleton and expression of immunophenotypic molecules of dendritic cells (DCs) were explored from the aspect of mechanical biology. Immature DCs (im DCs) and mature DCs (m DCs) were induced from CD14 ~ + monocytes by conventional methods. The DCs were loaded with shear force of 10 dyn / cm ~ 2 The changes of morphology and cytoskeleton of DCs were observed. The expression of CD80, CD83 and CD86 on the surface of DCs were detected by flow cytometry and real-time fluorescence quantitative PCR. Under the action of fluid shear stress, the diameter of DCs became smaller (p <0.05), and the cytoskeleton (F-actin) was obviously recombined. The expression of immunophenotyping molecules CD80, CD83 and CD86 on DCs at both protein and gene level was affected. DCs, therefore, are able to respond to physiological laminar flow shear stress, which may be a negative regulator of immune function in DCs, supporting “mechanoimmunology” and “Immunological Biology immunomechanobiology) ”, which is of great importance for understanding the immunomodulatory function of DCs.