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自发性节律搏动是原代单层培养心肌细胞的一个重要特征。培养心肌细胞的平均直径较小,自发性节律搏动频率较快。用玻璃微电极记录培养心肌细胞的电位,穿刺成功率较低,且难以稳定在细胞内。我们采用加硅涂层的玻璃微电极方法能在单层搏动培养心肌细胞内稳定地记录1h以上。 所用玻璃微电极是由内含玻璃纤维,外径为1mm的毛细管加热后,经两次拉曳,得到颈长15mm,尖端直径<0.1μm,长度为2～3μm的微电极。并在管内灌入3mol/L KCl溶液,直流电阻为30～50MΩ。在微电极灌注电解质液后,将其尖端浸在含2％二甲基二氯硅烷的四氯化碳溶液内硅化数秒钟。
Spontaneous rhythmic beating is an important feature of primary monolayer culture of cardiomyocytes. The average diameter of cultured cardiomyocytes is small, spontaneous rhythm beat faster. Using glass microelectrode to record the potential of cultured cardiomyocytes, the puncture success rate is low, and it is difficult to stabilize in the cells. We used a silicon-coated glass microelectrode method to stably record 1 h or more in a single layer of pulsatile cultured cardiomyocytes. The glass microelectrode used is a microelectrode with a length of 2 ~ 3μm with a neck length of 15mm, a tip diameter of <0.1μm and a length of 2 ~ 3μm after heated by a capillary tube containing glass fiber and an outer diameter of 1mm. And poured into the tube 3mol / L KCl solution, the DC resistance of 30 ~ 50MΩ. After the microelectrode was filled with the electrolyte solution, the tip was soaked in a carbon tetrachloride solution containing 2% dimethyldichlorosilane for several seconds.