论文部分内容阅读
为探讨流行性出血热(EHF)感染动物是否发病,以及EHF的血管病理变化和出血机制,本文通过EHF—V感染沙鼠和黑线姬鼠实验,观察内脏器官的病理改变。人工感染后14天于颈动脉放血处死,取心、肺、脑、肾、肝、胃肠组织作免疫荧光检测以及光镜电镜观察。并与三例EHF死亡患者的尸检材料做了对比研究。结果表明:感染鼠可以发病。主要病理改变为肺、肾、脑、肝的间质血管和微血管内皮细胞肿胀、退变、坏死脱落,管壁弹力纤维和嗜银纤维断裂,基底膜裸露。尤以肺、肾间质血管损伤较明显,器官的实质细胞有不同程度的变性坏死,但鼠的病变较人的为轻,且心、胃肠无明显病变,说明EHF的出血与血管壁的损害有明显关系。
To investigate the pathogenesis of epidemic hemorrhagic fever (EHF) infection in animals and the pathological changes and bleeding mechanism of EHF, the pathological changes of visceral organs were observed in EHF-V-infected gerbils and Apodemus agrarius. Fourteen days after artificial infection, the carotid arteries were sacrificed and the heart, lung, brain, kidney, liver and gastrointestinal tissues were taken for immunofluorescence and light microscopy. And compared with autopsy materials of three patients died of EHF. The results show that: infected mice can be disease. The main pathological changes of lung, kidney, brain, liver interstitial blood vessels and microvascular endothelial cells swelling, degeneration, necrosis, wall elastic fibers and silver fiber rupture, basement membrane bare. Especially in the lung and renal interstitium vascular injury is more obvious organ degeneration and necrosis of varying degrees of parenchymal cells, but the lesion in mice than human light, and the heart, gastrointestinal no significant pathological changes that EHF bleeding and vascular wall There is a clear relationship between damage.