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目的 从遗传性视网膜色素变性动物模型 rds小鼠中克隆视网膜色素变性发病过程中特异表达的基因。方法 应用差异显示技术 ,分析 rds小鼠发病过程中视网膜的 m RNA。对特异性表达的m RNA片段进行克隆测序。结果 在视网膜色素变性发病过程中 ,rds小鼠的视网膜存在着相当明显的基因表达差异。在所测序分析的 5个差异显示片段中 ,其中一个与 Gen Bank刚登录的功能未知的、从成年男性睾丸组织中克隆出来的 c DNA序列较高同源 (同一率为 86 % ) ,另外 4个为低同源序列。2 5天 rds小鼠高表达的一个差异片段 ,与 37天正常鼠表达而 rds小鼠不表达的另一个片段 ,长度相同 ,177个碱基只相差两个。结论 视网膜色素变性这类慢性病变 ,存在着多个基因的差异表达及调控
OBJECTIVE: To clone the gene specifically expressed in the pathogenesis of retinitis pigmentosa from rds mice of hereditary retinitis pigmentosa animal model. Methods Differential display technology was used to analyze the m RNA of the retina during the pathogenesis of rds mice. The specifically expressed m RNA fragments were cloned and sequenced. Results In the pathogenesis of retinitis pigmentosa, the rds mouse retina there is a considerable difference in gene expression. Of the five differential display sequences analyzed, one of the 5 differential display clones that Gen Bank had just registered had a higher homology (86% identity) to the c DNA sequence cloned from adult male testis and the other 4 were Low homologous sequence. A differentially expressed fragment of 25 days rds mice had the same length as the other fragment expressed in 37 days of normal mice but not in rds mice, with a difference of two in 177 bases. Conclusions Retinitis pigmentosa is a kind of chronic disease, there are many genes that are differentially expressed and regulated