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目的观察大鼠急性甲醇中毒后单核细胞趋化蛋白1(MCP-1)在大脑皮质的表达变化。方法应用通有混合气体(N2O/O2)的密闭有机玻璃箱并灌胃相应剂量甲醇溶液复制急性高剂量和低剂量甲醇中毒大鼠模型,运用Western Blot和免疫组化染色检测不同时间点大脑皮质中MCP-1的表达变化。结果 Western Blot显示急性甲醇中毒后低剂量组和高剂量组MCP-1蛋白表达在12 h明显升高(P<0.01),24 h达高峰,然后逐渐下降,3 d仍高于对照组(P<0.01)。高剂量组MCP-1蛋白表达在12 h和24 h高于低剂量组(P<0.05),3 d组MCP-1的表达无显著差异。免疫组化染色显示对照组大鼠大脑未见明显MCP-1阳性表达,急性甲醇中毒后,高剂量组24 h MCP-1阳性表达数增加,3 d达高峰,和对照组相比有显著差异(P<0.01),阳性表达多集中在梨状皮质,1周阳性反应减少,但与对照组相比仍有显著差异(P<0.01)。结论大鼠急性甲醇中毒后,脑组织MCP-1蛋白表达显著增高并持续一定时间,可能参与了脑组织病理变化过程,程度与甲醇中毒剂量有关。
Objective To observe the expression of monocyte chemoattractant protein 1 (MCP-1) in the cerebral cortex after acute methanol poisoning in rats. Methods Acute high-dose and low-dose methanol poisoning rat models were established by airtight plexiglass cartridges filled with mixed gas (N2O / O2) and the corresponding doses of methanol solution were orally administered. Western Blot and immunohistochemistry were used to detect the cerebral cortex at different time points MCP-1 expression changes. Results Western Blot showed that MCP-1 protein expression in low and high dose groups was significantly increased at 12 h (P <0.01) and peaked at 24 h after acute methanol poisoning, and then decreased gradually (P <0.01) <0.01). MCP-1 protein expression in high-dose group was higher than that in low-dose group at 12 h and 24 h (P <0.05), and there was no significant difference in MCP-1 expression at 3 d. Immunohistochemical staining showed that there was no significant MCP-1 expression in the brain of the control group. After acute methanol poisoning, the expression of MCP-1 in high-dose group increased at 24 h and reached the peak at 3 d, which was significantly different from the control group (P <0.01). The positive expression was mostly in piriform cortex, and the positive reaction decreased in one week, but there was still significant difference compared with the control group (P <0.01). Conclusion The expression of MCP-1 protein in brain tissue after acute methanol poisoning in rats is significantly increased and persists for a certain period of time, which may be involved in the process of pathological changes of brain tissue, the degree of which is related to the dose of methanol poisoning.