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前交叉韧带(ACL)损伤修复的研究进展表明,膝关节内覆在ACL上的滑膜组织对ACL损伤修复起到非常重要的作用。为探讨滑膜细胞对ACL成纤维细胞中影响组织修复的关键酶即赖氨酰氧化酶(LOXs)与基质金属蛋白酶(MMPs)表达及活性的影响,本文通过建立体外ACL成纤维细胞与滑膜细胞共培养体系,采用RT-PCR与明胶酶谱(zemography)技术定量分析比较单培养与共培养ACL成纤维细胞中LOXs与MMP-1、2、3基因的表达情况及MMP-2蛋白酶的活性。结果显示:(1)共培养促进ACL成纤维细胞中的LOXs与MMP-2的基因表达,但降低MMP-1、MMP-3的基因表达。(2)与单培养组相比,在不同时间点,共培养组都促进ACL成纤维细胞中MMP-2蛋白酶的表达及其活化程度。以上结果表明:ACL细胞和滑膜细胞之间存在密切的交流影响了LOXs与MMP-1、2、3的基因表达,为后续探索ACL损伤修复的机制及治疗方法提供了理论依据。
The research progress of anterior cruciate ligament (ACL) injury repair shows that the synovial tissue covered with ACL in the knee joint plays a very important role in ACL injury repair. To investigate the effect of synovial cells on the expression and activity of LOXs and MMPs in ACL fibroblasts, the effects of synovial cells on the proliferation of ACL fibroblasts, Cell co-culture system. The expression of LOXs and MMP-1,2,3 genes and the activity of MMP-2 protease in single cultured and co-cultured ACL fibroblasts were compared quantitatively by RT-PCR and zemography. The results showed that: (1) Co-culture promoted the gene expression of LOXs and MMP-2 in ACL fibroblasts, but decreased the gene expression of MMP-1 and MMP-3. (2) Compared with the single culture group, the co-culture group promoted the expression of MMP-2 protease in ACL fibroblasts and the degree of activation at different time points. The above results show that there is a close exchange between ACL cells and synoviocytes affecting the gene expression of LOXs and MMP-1, 2, which provides a theoretical basis for the subsequent exploration of the mechanism and treatment of ACL injury repair.