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目的观察硫化氢(H_2)对局灶性脑缺血损伤大鼠脑组织硫化氢/3-巯基丙酮酸转硫酶(H_2/3MST)及脑缺血体积的影响。方法健康成年雄性SD大鼠共80只,体重250~280 g,随机分为5组,每组16只,分别为假手术组;缺血模型组;NaHS低剂量组;Na HS中剂量组;NaHS高剂量组。NaHS低、中、高剂量组分别于大鼠脑缺血3 h时腹腔注射0.7、1.4和2.8 mg/kg的Na HS。5组大鼠均于缺血24 h断头取脑,TTC染色测定脑梗死灶体积,测定脑组织中H_2S含量,改良法测定脑组织3-巯基丙酮酸转硫酶(3MST)的活性。结果与缺血模型组比较,NaHS中、高剂量组大鼠脑梗死体积明显缩小,脑组织中H_2含量和3MST活性明显升高(P<0.05)。结论 NaHS中、高剂量可明显缩小脑缺血体积,增强脑组织3MST活性,升高脑组织H_2含量,表明NaHS对大鼠局灶性脑缺血损伤具有治疗作用。
Objective To observe the effects of hydrogen sulfide (H_2) on hydrogen sulfide / 3-thio-pyruvate-S-transferase (H_2 / 3MST) and cerebral ischemia volume in rats with focal cerebral ischemia. Methods A total of 80 healthy adult male Sprague-Dawley rats weighing 250-280 g were randomly divided into 5 groups (n = 16), sham operation group, ischemia model group, NaHS low dose group and Na HS medium dose group. NaHS high dose group. NaHS low, medium and high dose groups were intraperitoneally injected Na HS 0.7, 1.4 and 2.8 mg / kg at 3 h after cerebral ischemia. All rats in 5 groups were subjected to decapitation at 24 h after ischemia. TTC staining was used to determine the volume of cerebral infarction. The contents of H 2 S in brain tissue were determined. The activity of 3-mercaptopropionate S-transferase (3MST) in brain tissue was determined by modified method. Results Compared with the ischemic model group, the volume of cerebral infarction in NaHS medium and high dose groups was significantly reduced, while the content of H 2 and the activity of 3MST in brain tissues were significantly increased (P <0.05). Conclusion NaHS at medium and high doses can significantly reduce the volume of cerebral ischemia, enhance the activity of 3MST in brain tissue and increase the content of H 2 in brain tissue, indicating that NaHS has a therapeutic effect on focal cerebral ischemia in rats.