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人体棘球蚴病几乎在世界各地均有流行,有些地区细粒棘球蚴病和多房棘球蚴病共存,给鉴别诊断造成困难,严重危害人体健康。作者用酶联免疫吸附试验(ELISA)对来源于不同流行区的82例病人血清进行检测,鉴别诊断确诊率为95.1%,与检测同一流行区的病人血清的确诊率相同。证明虫株内和虫株间的变异不影响ELISA 的检测结果。在ELISA 中,同时采用两种诊断抗原Em1和Em2;其中,Em1为两种虫体所共有;Em2对多房
Human Echinococcosis is endemic in almost all parts of the world. In some areas, both Echinococcus granulosus and Echinococcoccus albopictus coexist, making it difficult to differentiate the diagnosis and seriously endangering human health. The authors used enzyme-linked immunosorbent assay (ELISA) to detect sera from 82 patients from different endemic areas, with a differential diagnosis of 95.1%, which was the same as the one used to detect patients in the same endemic area. Evidence of intra-strain and intra-strain variation did not affect ELISA test results. In the ELISA, two diagnostic antigens, Em1 and Em2, were used at the same time; Em1 was common to both species; Em2 was multifocal