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本研究建立了用测定血清睾酮的放免药盒直接测定唾液睾酮的方法。本法测定过程简单,样品无需提取,平均回收率为95.2%,批内和批间变异系数分别为10.8%和15%;最低可测浓度为6.5pg/ml。对40名健康男女运动员配对测定唾液睾酮和血清睾酮表明两者相关性很好(r=0.9068,P<0.001)。对两名健康成年男女不同天24小时唾液睾酮测定表明唾液睾酮具有明显的周期性节律。
In this study, we established a method for the direct determination of salivary testosterone using a radiopharmaceutical kit for measuring serum testosterone. The determination of the method is simple, the sample without extraction, the average recovery was 95.2%, intra-batch and inter-batch variation coefficient were 10.8% and 15%; the lowest measurable concentration of 6.5pg / ml. Pairwise determination of saliva testosterone and serum testosterone in 40 healthy male and female athletes showed good correlation (r = 0.9068, P <0.001). Twenty-four hours of testing for salivary testosterone in two healthy adult men and women on different days showed significant periodic rhythms in saliva testosterone.