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目的 研究肠缺血/再灌注(I/R)后氧自由基(ROS)对远隔器官肝脏的损伤作用,探讨亚甲蓝(MB)抗肝脏损伤的作用机制。方法 新西兰白兔32只,体重2.3～2.9 kg,随机分为4组,(1)正常组:本组仅做假手术处理。(2)I/R组:本组通过夹闭兔肠系膜前动脉1h复制肠I/R模型。(3)治疗A组:本组于松夹后静脉内给予MB 3 mg·kg-1。(4)治疗B组:本组于松夹后静脉内给予MB 15 mg·kg-1。颈总动脉置管,连续监测血压。于夹闭前、松夹即刻、松夹后30 min、1h和2h取血测定血中MDA浓度。实验结束后取一小块肝组织用于测定超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、黄嘌呤氧化酶(XO)及MDA。结果与I/R前基础值比较,I/R组血中MDA水平I/R后显著增加,同时血压显著下降;而治疗A组及B组血中MDA水平于I/R后均未显著增加。与正常组比较,I/R组肝组织MDA水平显著增高,而正常组与两个治疗组间肝组织中MDA无显著差异。结论 MB能减少肠I/R后ROS的生成并能对抗ROS对肝脏的损伤作用。
Objective To study the effect of oxygen free radicals (ROS) on the liver of remote organs after intestinal ischemia / reperfusion (I / R) and explore the mechanism of methylene blue (MB) against liver injury. Methods New Zealand white rabbits 32, weighing 2.3 ~ 2.9 kg, were randomly divided into 4 groups, (1) normal group: This group only sham surgery. (2) I / R group: The group was subjected to intestinal I / R model by clipping rabbit anterior mesenteric artery for 1 hour. (3) Treatment Group A: This group was intravenously administered with MB 3 mg · kg-1. (4) Treatment Group B: This group was intravenously administered with MB 15 mg · kg-1. Carotid artery catheterization, continuous monitoring of blood pressure. Immediately before the clamp, the clamps immediately after the clamp, 30 min after the clamp, 1h and 2h blood to determine MDA concentration in the blood. After the experiment, a small piece of liver tissue was taken for determination of superoxide dismutase (SOD), catalase (CAT), xanthine oxidase (XO) and MDA. Results Compared with the baseline value before I / R, the blood levels of MDA in I / R group increased significantly after I / R, meanwhile the blood pressure decreased significantly. However, the levels of MDA in I and R groups were not significantly increased after I / R . Compared with the normal group, the level of MDA in liver tissue of I / R group was significantly increased, while there was no significant difference in MDA between normal group and two treatment groups. Conclusion MB can reduce the production of ROS after intestinal I / R and can antagonize the injury of ROS on liver.