应用脂质体转染技术构建能表达肿瘤坏死因子－α（ＴＮＦ－α）基因的重组逆转录病毒。采用鱼精蛋白促进法，将重组病毒介导ＴＮＦ－α基因转染人脑胶质瘤浸润淋巴细胞（ＧＩＬ）。在体外，转染后的ＧＩＬ（ＴＮＦ－α／ＧＩＬ）的抗肿瘤活性比ＧＩＬ＋ＩＬ－２强２～５倍。在荷瘤裸鼠体内，ＴＮＦ－α／ＧＩＬ能显著抑制肿瘤生长。因此，ＴＮＦ－α基因转染的人脑胶质瘤浸润淋巴细胞（ＴＮＦ－α／ＧＩＬ）是脑胶质瘤免疫基因治疗的一种有效的效应细胞 Recombinant retrovirus expressing tumor necrosis factor-α (TNF-α) gene was constructed by lipofection technique. Recombinant virus-mediated TNF-α gene was transfected into human glioma infiltrating lymphocytes (GIL) by using protamine promoting method. In vitro, the transfection of GIL (TNF-α / GIL) antitumor activity than GIL + IL-2 stronger 2 to 5 times. In tumor-bearing nude mice, TNF-α / GIL can significantly inhibit tumor growth. Therefore, human glioma infiltrating lymphocytes (TNF-α / GIL) transfected with TNF-α gene is an effective effector cell for the gene therapy of glioma