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目的 监测肾移植前、后外周血白细胞淋巴细胞功能相关抗原１（ＬＦＡ１） 的表达水平，了解其变化规律， 探讨其在肾移植排斥反应中的意义。方法 应用单克隆抗体流式细胞仪荧光抗体技术监测肾移植受者外周血白细胞的ＬＦＡ１ 表达水平。结果 肾移植术后ＬＦＡ１ 表达水平较术前显著降低， 并维持在低水平， 发生排斥反应时显著升高； 排斥时的ＬＦＡ１ 表达水平与术后无排斥时及排斥反应控制后比较， 差异有显著性， 与术前比较， 差异无显著性。结论 ＬＦＡ１ 在白细胞上的表达与排斥反应密切相关； 监测ＬＦＡ１ 的表达水平有助于肾移植排斥反应的诊断。
Objective To monitor the expression of LFA1 in peripheral blood leukocytes before and after renal transplantation, and to investigate its variation and to explore its significance in renal allograft rejection. Methods Monoclonal antibody flow cytometry fluorescence antibody monitoring of peripheral blood leucocytes in renal transplant recipients LFA 1 expression levels. Results The expression of LFA-1 in renal transplantation was significantly lower than that before operation, and remained low at the time of rejection. The expression of LFA-1 at rejection was significantly higher than that at rejection and after rejection , The difference was significant, compared with preoperative, the difference was not significant. Conclusions The expression of LFA-1 on leukocytes is closely related to the rejection. Monitoring the expression of LFA-1 is helpful for the diagnosis of renal allograft rejection.