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【目的】探讨中药复方糖肾宝(主要由黄芪、大火草、葛根、莪术、三七组成)对高糖培养的大鼠系膜细胞(MCs)细胞外调节蛋白激酶1/2磷酸化(pERK1/2)及转化生长因子-β1(TGF-β1)、单核细胞趋化蛋白-1(MCP-1)表达的影响。【方法】体外培养大鼠MCs,随机分为正常组,高糖对照组,糖肾宝低、中、高剂量组(剂量分别为13.5、27、54 g/kg),厄贝沙坦组(剂量为25 mg/kg)。除正常组外,其余各组分别予以30 mmol/L葡萄糖+正常大鼠血清,糖肾宝低、中、高剂量血清,厄贝沙坦血清进行干预48 h。采用Western blot法检测细胞中pERK1/2水平、TGF-β1蛋白变化,RT-PCR法检测TGF-β1、MCP-1 mRNA表达,酶联免疫吸附法检测MCP-1蛋白和纤维连接蛋白(FN)表达。【结果】高糖对照组pERK1/2水平、TGF-β1、MCP-1、FN表达均显著高于正常组(P<0.05或P<0.01);药物干预的各组pERK1/2水平、TGF-β1、MCP-1、FN表达均显著低于高糖对照组(P<0.05或P<0.01);糖肾宝高剂量组pERK1/2水平显著低于厄贝沙坦组(P<0.01),TGF-β1、MCP-1、FN表达与厄贝沙坦组比较差异无统计学意义(P>0.05)。【结论】中药复方糖肾宝能抑制高糖培养的MCs细胞外基质的分泌,其机制可能与抑制ERK1/2磷酸化,并进而抑制TGF-β1和MCP-1表达有关。
【Objective】 To investigate the effect of Chinese herbal compound Tangshenbao (mainly composed of Astragalus, Firewood, Radix Puerariae, Rhizoma Curcumae and Radix Notoginseng) on extracellular regulation of protein kinase 1/2 phosphorylation (pERK1) in cultured rat mesangial cells (MCs) / 2) and the expression of transforming growth factor-β1 (TGF-β1) and monocyte chemoattractant protein-1 (MCP-1) 【Methods】 Rat MCs were cultured in vitro and were randomly divided into normal control group, high glucose control group, low and medium dosage of Tangshenbao, middle and high dosage of Ganxinbao (the dosage was 13.5, 27 and 54g / kg), irbesartan group The dose is 25 mg / kg). Except the normal group, the other groups were given 30 mmol / L glucose + normal rat serum, Tangshenbao low, middle and high dose serum, irbesartan serum for 48 h. The levels of pERK1 / 2 and TGF-β1 were detected by Western blot. The expressions of TGF-β1 and MCP-1 mRNA were detected by RT-PCR and the expressions of MCP-1 and fibronectin (FN) expression. 【Results】 The levels of pERK1 / 2 and the expressions of TGF-β1, MCP-1 and FN in high glucose control group were significantly higher than those in normal control group (P <0.05 or P <0.01) (P <0.05 or P <0.01). The levels of pERK1 / 2 in the high dose of Tangshenbao group were significantly lower than those in the irbesartan group (P0.01) The expression of TGF-β1, MCP-1 and FN in irbesartan group had no statistical difference (P> 0.05). 【Conclusion】 Tangshenbao can inhibit the secretion of extracellular matrix of MCs cultured in high glucose, which may be related to the inhibition of ERK1 / 2 phosphorylation and the inhibition of the expression of TGF-β1 and MCP-1.