论文部分内容阅读
以低温适应性能较好的“帅椒六号”甜椒为试材,设置(600±50)μL·L~(-1)(T1)与(1 200±50)μL·L~(-1)(T2)2个CO_2加富施肥处理,以不施用CO_2为对照(CK),门椒坐住后测定辣椒叶片光合生理指标SPAD值及Rubisco、RCA活性和耐低温性指标丙二醛(MDA)、可溶性糖含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性,研究了长期加富CO_2对甜椒光合生理和低温适应性的影响,以期为冬季在北方地区甜椒种植提供技术参考。结果表明:随着气温降低,各处理甜椒净光合速率、叶片SPAD值、叶片Rubisco和RCA活性均呈显著下降趋势。T2处理在处理30d后甜椒叶片净光合速率在各时段均显著高于CK和T1处理,较CK分别提高23.65%、37.51%、35.67%、16.86%、16.55%;处理60d后,T2净光合速率在11:00、13:00、15:00和17:00等4个时段均显著高于CK和T1处理,较CK分别提高33.16%、30.14%、16.97%、24.71%。T2处理在处理30、60d时,甜椒叶片SPAD值均显著高于CK。处理30d和60d,Rubisco活性均表现为T2处理显著高于CK和T1处理,分别比CK高15.8%、10.9%。RCA活性在处理30、60d均表现为T2处理均显著高于CK,比CK高9.96%、8.56%。不同处理下甜椒叶片SOD、POD和CAT活性表现为T2>T1>CK,T2处理显著高于CK和T1处理。不同处理下甜椒叶片丙二醛含量T2处理显著低于T1处理和CK。可溶性糖含量表现为T2>T1>CK,各处理差异达到显著水平。综上,在秋冬茬日光温室进行1 200μL·L~(-1) CO_2加富施肥可有效增强甜椒光合作用,提高甜椒在低温环境中的适应性,对于甜椒进行越冬栽培具有重要意义。
(600 ± 50) μL·L ~ (-1) (T1) and (1 200 ± 50) μL·L ~ (-1) of sweet pepper No. 6 with good low temperature adaptability, -1) (T2) Two CO_2 fertilization and fertilization treatments with no CO_2 as control (CK). The leaf photosynthetic parameters SPAD value, Rubisco, RCA activity and low temperature resistance index of malondialdehyde (MDA), soluble sugar content and superoxide dismutase (SOD) and catalase (CAT) activities of the peppers were studied. The effects of long-term CO 2 enrichment on photosynthetic physiology and low temperature adaptability of sweet peppers were studied. Sweet pepper planting provide technical reference. The results showed that the net photosynthetic rate, leaf SPAD value, Rubisco and RCA activities of leaves decreased significantly with the decrease of air temperature. Compared with CK, the net photosynthetic rate of sweet pepper leaves at T2 treatment was significantly higher than that of CK and T1 treatments at 30 d after treatment, which increased by 23.65%, 37.51%, 35.67%, 16.86% and 16.55% The rates were significantly higher than those of CK and T1 at 11: 00,13: 00,15: 00 and 17:00, which were 33.16%, 30.14%, 16.97% and 24.71% higher than CK. At the 30th and 60th days of T2 treatment, the SPAD value of sweet pepper leaves was significantly higher than that of CK. At 30d and 60d, Rubisco activity showed that T2 treatment was significantly higher than CK and T1 treatments, respectively, which were 15.8% and 10.9% higher than CK, respectively. The activities of RCA at T260 and T260 were all significantly higher than CK at 9 and 60 days of treatment, 9.96% and 8.56% higher than CK. Under different treatments, the activities of SOD, POD and CAT in leaves of sweet pepper showed that T2> T1> CK, T2 treatment was significantly higher than CK and T1 treatment. Under different treatments, the content of malondialdehyde in leaves of sweet pepper was significantly lower than that of T1 and CK. The content of soluble sugar showed T2> T1> CK, and the differences among treatments were significant. In summary, 1 200 μL·L -1 CO 2 enrichment and fertilization in autumn and winter stubble greenhouse can effectively enhance the photosynthesis of sweet pepper and improve the adaptability of sweet pepper in low temperature environment, which is of great significance for overwintering sweet pepper cultivation .