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大豆疫病是大豆重要病害之一,在世界范围内导致严重经济损失。防治大豆疫病最有效方法是利用抗病或耐病品种。筛选抗性资源是发掘抗性基因和抗病育种的基础。本研究鉴定了野生大豆资源对大豆疫病的抗病性和耐病性,以期发掘优异抗源。苗期用子叶贴菌块方法鉴定104份野生大豆资源对两个不同毒力的大豆疫霉分离物PSJS2(毒力型:1a,1b,1c,1d,1k,2,3a,3b,3c,4,5,6,7,8)和PS41-1(毒力型:1a,1d,2,3b,3c,4,5,6,7,8)抗性,结果表明33份资源抗PS41-1,35份资源抗PSJS2,其中18份抗两个分离物。在抗病性鉴定基础性上,用菌层接种方法对选择的82份资源进行耐病性鉴定,发现7份高耐病性资源。这些结果表明,野生大豆中可能含有新的大豆疫病抗病和(或)耐病资源,这些抗病或耐病资源可以用于未来大豆抗病育种,以丰富大豆对大豆疫病的抗性遗传基础。
Soybean disease is one of the major diseases of soybean and causes serious economic losses in the world. The most effective way to control the soybean disease is to use resistant or disease-resistant varieties. Screening for resistant resources is the basis for discovering resistance genes and resistance breeding. In this study, we identified the disease resistance and disease resistance of wild soybean to soybean disease in order to explore the excellent source of resistance. Seedlings of 104 soybean wild soybean cultivars were screened at the seedling stage for two isolates of PSJS2 (virulence: 1a, 1b, 1c, 1d, 1k, 2,3a, 3b, 3c, 4,5,6,7,8) and PS41-1 (virulence: 1a, 1d, 2,3b, 3c, 4,5,6,7,8). The results showed that 33 copies of the anti-PS41- 1, 35 parts anti-PSJS2, of which 18 were anti-two isolates. Based on the identification of disease resistance, 82 isolates were identified for pathogenicity by inoculation method and 7 high-disease-resistant resources were found. These results indicate that wild soybean might contain new resistance and / or disease-resistant resources to soybean disease, and these disease-resistant or disease-resistant resources could be used in future soybean disease-resistant breeding to enrich the genetic basis of soybean resistance to soybean disease.