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本实验用成年雄性Wistar大鼠37只,分为休克组、手术对照组和空白对照组。休克组大鼠在麻醉状态下,复制肠系膜上动脉闭塞性休克,并在小肠缺血1小时和1小时后再恢复小肠血流30分钟、90分钟等3个时间取材。手术对照组只重复休克组的手术操作而不阻断小肠血流,与休克组同时间取材。空白对照组不经任何处理,于麻醉后取材。取各组大鼠胃窦组织,制成4μm的连续切片。用免疫组织化学PAP法,在相邻切片上分别显示生长抑素细胞(D细胞)和胃素细胞(G细胞),并进行D和G细胞计数(个/mm~2粘膜)及计算G/D细胞比值。空白对照组D细胞计数为64.77±3.64,G细胞计数为98.54±6.52((?)±S(?)),G/D细胞比值为1.53±0.21((?)±S)。手术对照组的各项指标与空白对照组无显著差异(P>0.05)。休克组在恢复小肠血浣后30分钟和90分钟时,D细胞计数比相应手术对照组的分别下降了27.9％(P<0.05)和36.4％(P<0.01);G细胞计数则无显著差异(P>0.05);G/D细胞比值分别上升了45.1％(P<0.01)和38.5％(P<0.01)。结果提示,在肠系膜上动脉闭塞性休克过程中,大鼠胃窦粘膜D细胞数量的变化,反映了D细胞的分泌活动增强。G/D细胞比值上升,说明胃窦内分泌发生了变化。
In this experiment, 37 adult male Wistar rats were divided into shock group, operation control group and blank control group. In the anesthesia group, rats in shock group duplicated the superior mesenteric artery occlusive shock and recovered the small intestine blood flow for 30 minutes at 1 hour and 1 hour after intestinal ischemia, and then took it for 3 times at 90 minutes. The control group of the surgery only repeated the operation of the shock group without blocking the small intestine blood flow, taking the same time with the shock group. Blank control group without any treatment, drawn after anesthesia. Take the rat antral tissue, making 4μm serial sections. Using immunohistochemical PAP method, somatostatin cells (D cells) and gastrin cells (G cells) were respectively displayed on adjacent sections, and D and G cell counts (one / mm ~ 2 mucosa) and G / D cell ratio. The blank control group had a cell count of 64.77 ± 3.64, a G cell count of 98.54 ± 6.52 (±? S (?)) And a G / D ratio of 1.53 ± 0.21 (? ± S). There was no significant difference between the control group and the blank control group (P> 0.05). D-cell count decreased 27.9% (P <0.05) and 36.4% (P <0.01), respectively, in the shock group at 30 and 90 minutes after resuscitation, while there was no significant difference in G cell count (P> 0.05). The ratio of G / D cells increased by 45.1% (P <0.01) and 38.5% (P <0.01), respectively. The results suggest that changes in the number of D cells in the antral mucosa of the superior mesenteric artery in the process of occlusive shock reflect the enhanced secretory activity of D cells. G / D cell ratio increased, indicating changes in gastric endocrine.