人类新型β3半乳糖基转移酶基因β3GalT7的克隆和鉴定

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从人肺cDNA文库中克隆到人类β3-半乳糖基转移酶家族中的一个新成员β3GalT7(AY277592,EC2.4.1.-),并对其进行了鉴定.该基因定位在人类染色体19q13.2.它包含一个1191bp的开放阅读框(ORF).编码的蛋白质包括一个信号肽和一个半乳糖基转移酶结构域,分子质量和等电点分别为43.3ku和8.37.从原核表达中获得的融合蛋白的分子质量和预测相符.RNA印迹杂交显示β3GalT7在肺、喉和回肠组织中高表达,而在舌、乳房、子宫、睾丸等组织中表达较低.此外半定量RT-PCR显示β3GalT7在人类不同的肿瘤细胞中转录水平有明显差异.
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