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Ⅳ型胶原(CⅣ)是一种胶原蛋白,是构成细胞外基质的成分之一。近年来CⅣ用于肝纤维化、肝硬化的诊断报道较多,而用于肾脏疾病诊疗观察的研究则较少。本文通过测定肾脏疾病患者血清CⅣ的水平,探讨其与肾脏疾病之间的关系。 对象和方法 一、对象: (一)正常对照组:50例(男32,女18),为健康体检者,尿常规正常,年龄20～62岁(平均38.7岁)。 (二)肾病组:92例(男52,女40)。年龄14～71岁(平均49.6岁)。其中原发性肾小球肾炎32例;氮质血症30例;尿毒症30例。 二、方法:采集、分离正常人和各型肾病患者血清,-20℃保存待测。CⅣ放射免疫分析试剂盒由重庆肿瘤研究所提供。按说明书操作。统计学处理采用t检验。
Collagen type IV (CIV) is a collagen that forms one of the components of the extracellular matrix. In recent years, CⅣ for liver fibrosis, cirrhosis of the diagnosis of more reports, and for the diagnosis and treatment of kidney disease research is less. This article by measuring the serum levels of C Ⅳ in patients with kidney disease, to explore the relationship between the disease and the kidney. Subjects and methods First, the object: (a) normal control group: 50 cases (male 32, female 18), for physical examination, normal urine, aged 20 to 62 years (mean 38.7 years). (B) nephropathy group: 92 cases (male 52, female 40). Aged 14 to 71 years (mean 49.6 years). Including 32 cases of primary glomerulonephritis; 30 cases of azotemia; 30 cases of uremia. Second, methods: collection, separation of normal and various types of patients with kidney disease serum, -20 ℃ preserved for testing. C Ⅳ radioimmunoassay kit provided by the Chongqing Cancer Institute. Follow the instructions. Statistical analysis using t test.