论文部分内容阅读
建立了离子对高效液相色谱法测定无味红霉素含量。采用C8（5μm，4．0mm×125mm）柱（Lichrospher），以含0．1％（V／V）三乙胺的10mmol／L十二烷基硫酸钠溶液（用磷酸调pH3．4）-乙睛（52：48），检测波长为205nm。线性范围992～4958u／ml，r=0．9998（n=5），回收率100.3％，日内平均RSD为0．6％（n＝18），日间平均RSD为0.8％（n＝9）。
Ion-pair high performance liquid chromatographic determination of uric-free erythromycin was established. A 10 mmol / L sodium dodecyl sulfate solution (adjusted to pH 3.4 with phosphoric acid) containing 0.1% (V / V) triethylamine was loaded on a C8 (5 μm, 4.0 mm × 125 mm) column (Lichrospher) Eye (52:48), detection wavelength of 205nm. The average daily RSD was 0.6% (n = 18) and the average daytime RSD was 0.8% (n = 9) with a linear range of 992-4958u / ml, r = 0.9998 .