表柔红霉素是表阿霉素的半合成前体,通过中断dnmV基因,并将酮基还原酶aveBⅣ基因导入,可以将柔红霉素产生菌改造成表柔红霉素产生菌。在此基础上构建含有两个aveBⅣ表达单元的整合型质粒,并将其转入原有的表柔红霉素产生菌MHJ-02-30-1,使菌株内aveBⅣ的表达拷贝数由1个增加到3个。发酵验证表明,增加aveBⅣ基因拷贝数可以显著提高表柔红霉素的产量。目的菌株比出发菌株表柔红霉素的发酵单位提高了一倍以上。本文的工作为表柔红霉素发酵的产业化提供了有效途径。 Epirubicin is a semisynthetic precursor of epirubicin. The daunorubicin-producing bacterium can be transformed into epirubicin-producing bacterium by interrupting the dnmV gene and introducing the aveBIV gene of ketoreductase. On this basis, an integrated plasmid containing two aveBIV expression units was constructed and transformed into the original epidermidis erythromycin producing strain MHJ-02-30-1, so that the expression copy number of aveBIV in the strain was changed from 1 Increase to three. Fermentation tests showed that increasing aveBⅣ gene copy number significantly increased the yield of epirubicin. The target strains than the original strain daunomycin fermentation unit more than doubled. The work in this paper provides an effective approach to the industrialization of epidermidin fermentation.