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为了研究金环蛇抗菌肽Cathelicidin-BF对临床常见菌株的体外抗菌活性和细菌是否容易对其产生耐药,分别通过琼脂平板稀释法和肉汤稀释法测定Cathelicidin-BF的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。研究接种量与pH值对其MIC的影响和本品对细菌生长增殖的抑制作用,利用耐受诱导实验研究细菌对本品是否易产生耐药。结果表明金环蛇抗菌肽Cathelicidin-BF对革兰阳性菌和阴性菌都有抑制作用,MIC低至2μg/mL,在用药处理的10 h内未见细菌生长,且在耐药性诱导实验期间,细菌对Cathelicidin-BF没有产生耐药。金环蛇抗菌肽Cathelicidin-BF具有广谱抗菌活性,且与抗生素相比不易产生耐药,提示其可作为治疗感染性疾病的候选药物。
In order to study the antibacterial activity of Cathelicidin-BF on clinical antibacterial activity in vitro and the susceptibility of bacteria to it, the minimal inhibitory concentrations (MICs) of Cathelicidin-BF were determined by agar plate dilution and broth dilution ) And minimum bactericidal concentration (MBC). Inoculation volume and pH value of the MIC study of its impact on bacterial growth and inhibition of the use of tolerance induced experiment to study the bacteria susceptible to the goods. The results showed that the antimicrobial peptide Cathelicidin-BF inhibited both Gram-positive and -negative bacteria with a MIC as low as 2 μg / mL. No bacterial growth was observed within 10 h after drug treatment and during the drug-resistance induction experiment Bacteria did not develop resistance to Cathelicidin-BF. The antibacterial peptide Cathelicidin-BF has broad-spectrum antibacterial activity and is not easily drug-resistant compared with antibiotics, suggesting that it can be used as a candidate drug for the treatment of infectious diseases.