本实验观察了冷冻损伤后大鼠血清中血管紧张素Ⅰ转换酶(ACE)活性的改变及其与冷冻损伤程序度的关系。150只Wistar大鼠乙醚麻醉后双后足在-25℃乙醇中浸冻至组织温度分别达到-20℃、-10℃和0℃,冻后4小时、24小时、3天、5天和7天用紫外分光光度法测定血清中ACE活性。结果表明,冻后大鼠血清中ACE活性呈不同程度的增加,其增加程度与冷冻损伤程度有关。提示冷冻损伤可引起不同程度的血管内皮细胞损伤,使之释放到血流中的ACE活性增加。 In this study, the changes of serum ACE activity and the degree of freezing injury were observed after freezing injury. 150 Wistar rats were anesthetized with ether and dipped into double tibias at -25 ° C in ethanol until tissue temperatures reached -20 ° C, -10 ° C and 0 ° C, 4 hours, 4 hours, 3 days, 5 days and 7 days after freezing Determination of serum ACE activity by UV spectrophotometry. The results showed that the serum ACE activity in frozen rats increased to varying degrees, the degree of increase related to the degree of freezing injury. Tip frozen injury can cause varying degrees of vascular endothelial cell damage, so that the release of ACE activity into the bloodstream increased.