Objective: Several in vivo studies suggested that osteogenic response of bone saturated with continued long-term mechanical loading,but the saturation could be reversed with rest-inserted loading.This suggests that recovery periods would restore bones sensitivity to mechanical stimuli.Furthermore,it has been demonstrated that rest-inserted loading was able to promote mechanosensitivity of osteoblast.As the putative mechanosensors,osteocytes orchestrate bone remodeling by transducing mechanical signal into biological signal.However,the effect of rest-inserted loading on osteocyte remains unclear.The aim of this study was to investigate the effect of rest-inserted loading on the mechanosensitivity of osteocytes.