The single-molecule real-time (SMRT) sequencing platform presented by Pacific Biosciences (PacBio) is regarded as the third-generation sequencing technology.PacBio offers long reads from several to tens of kilobases (Kb),ideal for filling unsequenced genomic gaps due to unusual sequence contexts (i.e.,high-GC or repeat-rich DNAs).PacBios long-read property is also favorable for detecting large DNA fragment harboring structural variations (SVs),especially inversions,translocations,and large insertions/deletions (indels).