【摘 要】
：
MicroRNAs (miRNAs),i.e.small non-coding RNA molecules (～22 nt),can bind to one or more target sites on a gene transcript to negatively regulate protein expressio
【机 构】
：
DepartmentofBiologicalScienceandTechnology,InstituteofBioinformaticsandSystemsBiology,NationalChiaoT
【出 处】
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The 4th International Conference of Genomics Beyond Biologic
论文部分内容阅读
MicroRNAs (miRNAs),i.e.small non-coding RNA molecules (～22 nt),can bind to one or more target sites on a gene transcript to negatively regulate protein expression,subsequently controlling many cellular mechanisms.High-throughput sequencing technology enables a revolutionary advance to identifying miRNA-target interactions,transcription factor (TF)-miRNA regulations,and subsequently to reconstructing TFmiRNA-target regulatory networks.Chromatin immunoprecipitation sequencing (ChIPseq) can be utilized for transcriptional regulation of miRNAs.High-throughput experiments (CLIP-seq,PAR-CLIP-seq and CLASH-seq) are powerful methods for identifying miRNA-target interactions.
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