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目的评价改良同步化染色体培养技术在脐血产前诊断核型分析中的应用。方法选择2011年6月-2012年12月就诊的1 200例有产前诊断指征的孕妇,抽脐血进行产前诊断,用改良同步化染色体培养技术与常规法进行核型分析。结果培养成功率均为100.00%。改良同步化染色体培养技术染色体核型达到500~550条带,常规法染色体核型为320~400条带。改良同步化染色体培养技术的染色体长度及分辨率明显提高。对1 200份标本进行核型分析,检出染色体数目、结构异常105例(8.75%),其中染色体结构异常69例(5.75%),染色体数目异常36例(3.00%)。结论改良同步化染色体培养技术操作简单,方法稳定,获得的分裂相比较分散,核型分辨率可提高至500~550条带,应用于脐血产前诊断染色体核型分析有利于发现更多的染色体结构异常,在产前诊断中具有重要的临床价值。
Objective To evaluate the application of modified synchronous chromosome culture technique in karyotype analysis of prenatal diagnosis of umbilical cord blood. Methods A total of 1,200 pregnant women with prenatal diagnosis indications from June 2011 to December 2012 were selected for prenatal diagnosis by umbilical cord blood and karyotype analysis by conventional synchronized chromosome culture technique. Results The successful rate of culture was 100.00%. Improved synchronous chromosome culture technology chromosome karyotype reached 500 to 550 bands, the conventional method karyotype 320 to 400 bands. Chromosomal length and resolution of the improved synchronized chromosome cultivation technique were significantly improved. Karyotype analysis of 1 200 specimens showed that there were 105 (8.75%) chromosomal abnormalities in chromosome number, 69 (5.75%) chromosomal aberrations and 36 (3.00%) chromosomal abnormalities. Conclusion The improved synchronized chromosome culture technique is simple and stable in method. The obtained split is relatively fragmented and the resolution of karyotype can be increased to 500-550. The prenatal diagnosis of karyotype in cord blood is helpful to find out more Chromosome structural abnormalities in prenatal diagnosis has important clinical value.