Background: Physiological functions of endogenous hydrogen sulfide (H2S), as a neuromodulator in central nervous system attract much attention recently.Previous studies clue to us that H2S plays important roles in regulating gastric function within the gastroenteric centrum of medulla (dorsal motor nucleus of the vagus, nucleus ambiguus, nucleus of solitary tract and area postrema).The aim of this study is to investigate the effects of microinjection of sodium hydrogen sulfide (NaHS), exogenous hydrogen sulfide donor, on gastric motility in rats.Material and methods: An aqueous capsule connected with a pressotransducer was inserted into the pylorus through the forestomach.The gastric motility was recorded by the RM6240 Biological function experiment system continuously.The amplitude, duration, frequency, and motility index of gastric contraction waves within 15 minutes before microinjection and after microinjection were measured.Results: Microinjection of NaHS (0.04mol, μL, n=3) into the right dorsal motor nucleus of the vagus (DMV) significantly inhibited gastric motility in Wistar rats.Total amplitude of contraction waves decreased from 157.30± 26.33 (before microinjection) to 95.50 ± 15.36 mm/5min (P<0.05), gastric motility index decreased from 1005.80 ± 195.03 (before microinjection) to 723.50 ± 150.17 (P<0.05) after NaHS was microinjected into DMV.Conclusions: The data of these experiments suggest that microinjection of NaHS into DMV inhibited the gastric motility obviously.