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Damage accumulation deriving from load cycles is one of the most important features in the vulnerability assessment of historical bridge steel structures (Sustainable Bridges,2007; JRC,B.Kiihn,2008).The old deterministic approaches in the current regulations (Italian Ministry of Infrastructure,2008) have been replaced by a new fatigue analysis probabilistic approach.Starting from Wohler curves and referring to Eurocodes prescriptions (Eurocode 3,1993) a vulnerability analysis has been performed by estimating the structure probability to be damaged over time.Performing a structural vulnerability analysis means to evaluate the safety of a structure assessing its propensity to be damaged by the loads.