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Aim of the study:Reduction of Sheng-Nao-Kang decoction (RSNK),composed of Salvia miltiorrhiza Bge.,Ligusticum chuanxiong Hort.,Astragalus membranaceus (Fisch.) Bunge.,Pueraria lobata (Willd.) Ohwi.,Paeonia lactiflora Pall.and Panax notoginseng (Burk.) F.H.Chen.,is a modified traditional Chinese medicinal formula of Sheng-Nao-Kang pill preparation,which has been investigated its protective effect on focal cerebral ischemia-reperfusion injury in rat in our previous report.To evaluate the antithrombotic effect of RSNK in blood stasis model rats and explore the potential mechanisms.Materials and methods:Ice water bath and subcutaneous injection of norepinephrine and bovme serum albumin were both used to establish the acute blood stasis rat model.The anticoagulant activities were investigated by measuring activated partial thromboplastin time (APTT),thrombin time (TT),prothrombin time (PT),and the content of fibrinogen (FIB).At the meanwhile,the levels of thromboxane A2 (TXA2) and prostaglandins I2 (PGI2) in serum were measured and endothelial nitric oxide synthase (eNOS) and endothelin (ET) in plasma were also detected.Results:The treatment of RSNK was able to prolong APTT,TT and PT,and decrease FIB content obviously.Furthermore,RSNK administration markedly suppressed TXB2 level and up-regulated 6-keto-PGFla level of the blood-stasis model rats,accompanied with the decrease of T/K (P<0.05).The level of ET and TXA2 in plasma was down-regulated and the levels of eNOS in plasma and PGI2 in serum was up-regulated in RSNK-treated rats compared with model rats (P<0.05).The effect of RSNK administration in the dosage of 2.8 g/kg was better.Conclusion:The results of the present study suggest that RSNK possesses remarkable antithrombotic properties,affecting platelet aggregation functions and anticoagulation activities.